Plasmid P1 replication: negative control by repeated DNA sequences.
نویسندگان
چکیده
منابع مشابه
Multiple homeostatic mechanisms in the control of P1 plasmid replication.
Many organisms control initiation of DNA replication by limiting supply or activity of initiator proteins. In plasmids, such as P1, initiators are limited primarily by transcription and dimerization. However, the relevance of initiator limitation to plasmid copy number control has appeared doubtful, because initiator oversupply increases the copy number only marginally. Copy number control inst...
متن کاملCritical sequences in the core of the P1 plasmid replication origin.
The core of the P1 plasmid replication origin consists of a series of 7-bp repeats and a G+C-rich stretch. Methylation of the GATC sequences in the repeats is essential. Forty different single-base mutations in the region were isolated and assayed for origin function. A single-base change within any 7-bp repeat could block the origin, irrespective of whether GATC bases were affected. The repeat...
متن کاملDirect primer walking on P1 plasmid DNA.
The development of vectors such as P1, P1-derived artificial chromosome (PAC) or bacterial artificial chromosome (BAC) (2,6,8) has permitted the cloning of large DNA fragments. These vectors have become common in largescale sequencing and mapping projects. Therefore, a reliable primer walking protocol for P1 plasmids is desirable. It has been demonstrated previously that sequencing of P1 plasmi...
متن کاملPersistence of repeated sequences that evolve by replication slippage.
The evolution of short repeated sequences by replication slippage under the assumption of selective neutrality is modeled using a linear birth and death process. The equilibrium distribution, the distribution of the life expectancy of a repeated sequence when the process starts from two repeats, the age distribution of repeats, the probability of obtaining two genes with i and j copies which di...
متن کاملNegative control of plasmid R6K replication: possible role of intermolecular coupling of replication origins.
The gamma origin binding sites of the replication initiator pi protein, composed of seven 22-base-pair (bp) direct repeats and previously shown to be essential for replication of plasmid R6K, can also act as an inhibitor of R6K replication in Escherichia coli cells if provided in trans. Inhibition is dependent upon the ability of these repeats to bind the R6K-encoded pi protein but is not overc...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1984
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.81.20.6456